سلام

از این کد استفاده کن
Private Sub Button1_Click(sender As Object, e As EventArgs) Handles Button1.Click
Dim bekhoon As New System.IO.StreamReader(Application.StartupPath & "\file.txt", True)
While bekhoon.Peek > -1
Dim khoondam() As String = bekhoon.ReadLine.Split("/")
TextBox1.Text = khoondam(0)
TextBox2.Text = khoondam(1)
End While
bekhoon.Close()
End Sub