سلام ،
برای اینکار میتوانید از پروپرتی Expression مربوط به شی ء DataColumn استفاده نمایید ،
مثالی از MSDN :

private void CalcColumns()
{
DataTable table = new DataTable ();

// Create the first column.
DataColumn priceColumn = new DataColumn();
priceColumn.DataType = System.Type.GetType("System.Decimal");
priceColumn.ColumnName = "price";
priceColumn.DefaultValue = 50;

// Create the second, calculated, column.
DataColumn taxColumn = new DataColumn();
taxColumn.DataType = System.Type.GetType("System.Decimal");
taxColumn.ColumnName = "tax";
taxColumn.Expression = "price * 0.0862";

// Create third column.
DataColumn totalColumn = new DataColumn();
totalColumn.DataType = System.Type.GetType("System.Decimal");
totalColumn.ColumnName = "total";
totalColumn.Expression = "price + tax";
// Add columns to DataTable.
table.Columns.Add(priceColumn);
table.Columns.Add(taxColumn);
table.Columns.Add(totalColumn);
DataRow row = table.NewRow();
table.Rows.Add(row);
DataView view = new DataView(table);
dataGrid1.DataSource = view;
}